HMGB1, His, Human

ArtNr	Z02803-50
Hersteller	GenScript
Menge	50 ug
Quantity options	1 mg 50 ug
Kategorie	Oncology Neuroscience Neuroinflammation Aging & Neurological Disorders By Cell Type Peptides & Proteins Recombinant Proteins Adenocarcinoma
Typ	Proteins
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Specific against	Human (Homo sapiens)
Purity	>95% by SDS-PAGE and HPLC analyses.
Sequence	MGKGDPKKPR, GKMSSYAFFV, QTCREEHKKK, HPDASVNFSE, FSKKCSERWK, TMSAKEKGKF, EDMAKADKAR, YEREMKTYIP, PKGETKKKFK, DPNAPKRPPS, AFFLFCSEYR, PKIKGEHPGL, SIGDVAKKLG, EMWNNTAADD, KQPYEKKAAK, LKEKYEKDIA, AYRAKGKPDA, AKKGVVKAEK, SKKKKEEEED, EEDEEDEEEE, EDEEDEDEEE, DDD
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02803-High-Mobility_Group_Box_1_HMGB1_His6-tagged_Human, Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.</td></tr><tr><th>M.W.</th><td colspan="7"> Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids.</td></tr><tr><th>Purity</th><td colspan="7"> >95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.2EU/ug of rHuHMGB1 as determined by LAL method.</td></tr><tr><th>Storage</th><td colspan="7"> This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized from a 0.2um filtered concentrated, solution in PBS, pH 7.4.</td></tr><tr><th>Reconstitution</th><td colspan="7"> We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions.</td></tr><tr><th>Physical Appearance</th><td colspan="7"> Sterile Filtered White lyophilized (freeze-dried) powder.</td></tr><tr><th>Usage</th><td colspan="7"> This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.</td></tr><tr><th>Sequence</th><td colspan="7"> MGKGDPKKPR, GKMSSYAFFV, QTCREEHKKK, HPDASVNFSE, FSKKCSERWK, TMSAKEKGKF, EDMAKADKAR, YEREMKTYIP, PKGETKKKFK, DPNAPKRPPS, AFFLFCSEYR, PKIKGEHPGL, SIGDVAKKLG, EMWNNTAADD, KQPYEKKAAK, LKEKYEKDIA, AYRAKGKPDA, AKKGVVKAEK, SKKKKEEEED, EEDEEDEEEE, EDEEDEDEEE, DDDDELEHHH, HHH</td></tr>
Similar products	High-Mobility
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids.
Product Line	Other Proteins
Description	Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2um filtered concentrated, solution in PBS, pH 7.4.
Endotoxin Level	Less than 0.2EU/ug of rHuHMGB1 as determined by LAL method.
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

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Z02803-50-datasheet.pdf

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