M-CSF, Human

ArtNr	Z02914-1
Hersteller	GenScript
Menge	1 mg
Quantity options	1 mg 10 ug 100 ug
Kategorie	Neuroscience Metabolism Diabetes Molecular Targets for Neuroscience Aging & Neurological Disorders Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Cell Metabolism Cardiovascular Research Proliferation Oxidative Stress Hypertension
Typ	Proteins
Specific against	Human (Homo sapiens)
Purity	> 95% as analyzed by non-reducing SDS-PAGE.
Sequence	Glu33-Ser190(accession number: P09603, Isoform 3),expressed with an N-terminal Met.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02914_1-Macrophage_Colony_Stimulating_Factor_M_CSF_Human,Macrophage Colony-Stimulating Factor 1 (M-CSF) , involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63. Recombinant human, Macrophage Colony-Stimulating Factor 1 (rhM-CSF), produced in, E. coli, is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 28 kDa, observed by non-reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% as analyzed by non-reducing SDS-PAGE.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 106 units/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant human, Macrophage Colony-Stimulating Factor 1 (rhM-CSF), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4C or up to 3 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Glu33-Ser190(accession number: P09603, Isoform 3),expressed with an N-terminal Met.</td></tr>
Similar products	Macrophage
Versandbedingung	Gekühlt
Lieferbar
Specificity	ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 106 units/mg.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	28 kDa, observed by non-reducing SDS-PAGE.
Product Line	Growth Factors
Manufacturer - Specificity	ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 106 units/mg.
Description	Macrophage Colony-Stimulating Factor 1 (M-CSF) , involved especially in monocytopoiesis, [1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63. Recombinant human, Macrophage Colony-Stimulating Factor 1 (rhM-CSF), produced in, E. coli, is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
Endotoxin Level	<1 EU/ug, determined by LAL method.

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