MEC/CCL28, Mouse

ArtNr	Z02857-1
Hersteller	GenScript
Menge	1 mg (2x500 ug)
Quantity options	1 mg (2x500 ug) 20 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Migration & Invasion
Typ	Proteins
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Specific against	Mouse (Murine, Mus musculus)
Purity	>97% by SDS-PAGE and HPLC analyses.
Sequence	SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKRRRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHRTRGTHRHEAS R
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02857-MEC_CCL28_Mouse, Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag1 mouse kidney cDNA library. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.</td></tr><tr><th>M.W.</th><td colspan="7"> 12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.</td></tr><tr><th>Purity</th><td colspan="7"> >97% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.2EU/ug of rMuMEC/CCL28 as determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 10 ng/ml, corresponding to a specific activity of &
Similar products	MEC/CCL28
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Specificity	Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 10 ng/ml, corresponding to a specific activity of ＞, 1.0 x 105 IU/mg.
Country of Origin	USA
Storage Conditions	This lyophilized preparation is stable for several weeks at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.
Molecular Weight	12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.
Product Line	Cytokine, Chemokines & Growth Factors
Manufacturer - Specificity	Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 10 ng/ml, corresponding to a specific activity of ＞, 1.0 x 105 IU/mg.
Description	Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag1 mouse kidney cDNA library. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2µ, m filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Endotoxin Level	Less than 0.2EU/ug of rMuMEC/CCL28 as determined by LAL method.
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

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Menge: 1 mg (2x500 ug)

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