TIGIT Fc Chimera, Human

USA

Lyophilized recombinant TIGIT Fc Chimera, Human remains stable for up to 6 months at -80C from date of receipt. Upon reconstitution, Human TIGIT Fc Chimera should be stable for up to 1 week at 4C or up to 3 months at -20C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3). It belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). It binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells.
Recombinant Human TIGIT Fc Chimera produced in HEK293 cells is a polypeptide chain containing 352 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhTIGIT has a molecular mass of 50-55 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.