MOUSE ANTI HUMAN HEXOKINASE 1

HEXOKINASE 1

Purified IgG prepared by affinity chromatography on Protein G

Specifically recognises Hexokinase 1 a 102kDa major allosteric enzyme of the glycolytic pathway which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P). Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type I to IV) which is expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. Recommended Positive Controls: HEPG2 CELL TOTAL PROTEIN LYSATERecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGHuCAL Anti Mouse IgG2aRabbit Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMGoat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)

Carbohydrate metabolism; hexose metabolism. Subunit structureMonomer. Ref. 13Ref. 15Subcellular locationMitochondrion outer membrane.

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. Involvement in diseaseDefects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature. Ref. 17Ref. 18Miscellaneous: In vertebrates there are four major glucose-phosphorylating isoenzymes designated hexokinase I II III and IV (glucokinase). Sequence similaritiesBelongs to the hexokinase family. 1. Moss S. E. and Morgan R. O. (2004) The annexins. Genome Biol. 5: 219-219. 8 [1] \" Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase. \" Huang K. -S. Wallner B. P. Mattaliano R. J. Tizard R. Burne C. Frey A. Hession C. McGray P. Sinclair L. K. Chow E. P. Browning J. L. Ramachandran K. L. Tang J. Smart J. E. Pepinsky R. B. Cell 46:191-199(1986) [PubMed: 3013422] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta. [2] \" Characterization of the human lipocortin-2-encoding multigene family: its structure suggests the existence of a short amino acid unit undergoing duplication. \" Spano F. Raugei G. Palla E. Colella C. Melli M. Gene 95:243-251(1990) [PubMed: 2174397] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). [3] \" Cloning of human full-length CDSs in BD Creator(TM) system donor vector. \" Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. LaBaer J. Lin Y. Phelan M. Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). [4] \" The full-ORF clone resource of the German cDNA consortium. \" Bechtel S. Rosenfelder H. Duda A. Schmidt C. P. Ernst U. Wellenreuther R. Mehrle A. Schuster C. Bahr A. Bloecker H. Heubner D. Hoerlein A. Michel G. Wedler H. Koehrer K. Ottenwaelder B. Poustka A. Wiemann S. Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon endothelium. [5] \" Analysis of the DNA sequence and duplication history of human chromosome 15. \" Zody M. C. Garber M. Sharpe T. Young S. K. Rowen L. O\' Neill K. Whittaker C. A. Kamal M. Chang J. L. Cuomo C. A. Dewar K. FitzGerald M. G. Kodira C. D. Madan A. Qin S. Yang X. Abbasi N. Abouelleil A. Arachchi H. M. Baradarani L. Birditt B. Bloom S. Bloom T. Borowsky M. L. Burke J. Butler J. Cook A. DeArellano K. DeCaprio D. Dorris L. III Dors M. Eichler E. E. Engels R. Fahey J. Fleetwood P. Friedman C. Gearin G. Hall J. L. Hensley G. Johnson E. Jones C. Kamat A. Kaur A. Locke D. P. Madan A. Munson G. Jaffe D. B. Lui A. Macdonald P. Mauceli E. Naylor J. W. Nesbitt R. Nicol R. O\' Leary S. B. Ratcliffe A. Rounsley S. She X. Sneddon K. M. B. Stewart S. Sougnez C. Stone S. M. Topham K. Vincent D. Wang S. Zimmer A. R. Birren B. W. Hood L. Lander E. S. Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1) VARIANT LEU-98. Tissue: Brain Colon Pancreas Prostate Skin Testis and Urinary bladder. [7] Bienvenut W. V. Murray L. Brunton V. G. Frame M. C. Bensaad K. /a> Vousden K. H. Submitted (FEB-2008) to UniProtKBCited for: PROTEIN SEQUENCE OF 2-10; 38-47; 50-63; 69-77; 89-115; 153-169 AND 213-220 CLEAVAGE OF INITIATOR METHIONINE ACETYLATION AT SER-2 MASS SPECTROMETRY. Tissue: Colon adenocarcinoma and Osteosarcoma. [8] \" The protein-tyrosine kinase substrate calpactin I heavy chain (p36) is part of the primer recognition protein complex that interacts with DNA polymerase alpha. \" Jindal H. K. Chaney W. G. Anderson C. W. Davis R. G. Vishwanatha J. K. J. Biol. Chem. 266:5169-5176(1991) [PubMed: 1825830] [Abstract]Cited for: PROTEIN SEQUENCE OF 11-27; 49-62; 68-77; 120-135; 314-323 AND 329-339. [9] \" Identification and characterization of alpha-protein kinase C binding proteins in normal and transformed REF52 cells. \" Hyatt S. L. Liao L. Chapline C. Jaken S. Biochemistry 33:1223-1228(1994) [PubMed: 8110754] [Abstract]Cited for: PROTEIN SEQUENCE OF 15-40 AND 50-63. [10] \" An endothelial cell-surface form of annexin II binds human cytomegalovirus. \" Wright J. F. Kurosky A. Wasi S. Biochem. Biophys. Res. Commun. 198:983-989(1994) [PubMed: 8117306] [Abstract]Cited for: PROTEIN SEQUENCE OF 18-37; 119-126; 172-191 AND 301-307 INTERACTION WITH HCMV. Tissue: Umbilical vein endothelial cell.