Cytokeratin 4

GWB-B50EC9

A synthetic peptide corresponding to residues near the N-terminus of human CK-4 was used as an immunogen.

WB: 1:2000IHC: 1:100 - 250ICC: 1:100 - 250. Intermediate filaments are protein polymers which together with actin filaments and microtubules form the cytoskeleton of cells. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Expression of the genes encoding these stratification-related cytokeratins starts already in the basal cell layer and does not depend on vertical differentiation and detachment from the basal lamina. Results also show that simple epithelial and stratification-related cytokeratins can be coexpressed in basal cell layers of certain stratified epithelia such as esophagus (1). Mucous keratin CK4 is known to appear in epidermis under certain abnormal conditions. a dose-response study showed that the de novo appearance of K4 can be utilized as a sensitive test for retinoid bioactivity in epidermis in vivo (2). 1. Leube RE et al. J Cell Biol 106(4):1249-61 19882. Virtanen M et al. J. Invest Dermatol 114(3):487-93 20003. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487