AMPK alpha 2

ArtNr	18-272-197264
Hersteller	GENWAY
Menge	0.05 ml
Kategorie	Oncology Metabolism Antibodies By Cell Type Hematology Lymphoma Cell Biology Obesity Cell Status Immunoprecipitation Western Blot Autophagy Primary Antibodies
Typ	Antibody
Applikationen	WB, IP
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-D936D0
Similar products	18-272-197264
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Genway ID:	GWB-D936D0
Isotype:	IgG
Immunogen:	Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase alpha-2 catalytic subunit encoded within exon 7.
Antigen Species:	Human
Target:	AMPK alpha 2
Concentration:	1. 0 mg/ml Storage
Buffer:	0. 1ml Tris-citrate/phosphate pH 7-8 with 0. 1% sodium azide
Application Note:	For IP: Use at a concentration of 2 - 8 µ g/ml. For WB: Use at a dilution of 0. 2 - 2µ g/ml. Predicted
Molecular Weight:	63 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. The AMP-activated protein kinase (AMPK) in mammals and its homologue in Saccharomyces cerevisiae are activated by cellular stresses associated with ATP depletion. AMPK is a heterotrimer comprising a catalytic alpha subunit with associated beta and gamma subunits. The alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their AMP-dependence and subcellular localization with alpha 2 complexes being partly nuclear. AMPK is activated allosterically by 5\' -AMP which also promotes phosphorylation and activation by an upstream kinase and inhibits dephosphorylation and inactivation. Elevation of AMP always accompanies depletion of ATP due to the action of adenylate kinase. Since high ATP antagonizes the activating effects of AMP the system behaves like a cellular \' fuel gauge\' . It is activated by various types of stress associated with ATP depletion such as hypoxia heat shock metabolic poisoning and in muscle exercise. AMPK phosphorylates multiple targets which switch off anabolic pathways and switch on alternative catabolic pathways.
Function:	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5\' -AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Catalytic Activity:	ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium.
Enzyme Regulation:	Binding of AMP results in allosteric activation inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions without detectable changes in the AMP/ATP ratio (By similarity).
Subunit:	Heterotrimer of a catalytic subunit a beta and a gamma non-catalytic subunits.
Similarity:	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Similarity:	Contains 1 protein kinase domain.

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