AMPK alpha 2

GWB-D936D0

Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase alpha-2 catalytic subunit encoded within exon 7.

AMPK alpha 2

0. 1ml Tris-citrate/phosphate pH 7-8 with 0. 1% sodium azide

63 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. The AMP-activated protein kinase (AMPK) in mammals and its homologue in Saccharomyces cerevisiae are activated by cellular stresses associated with ATP depletion. AMPK is a heterotrimer comprising a catalytic alpha subunit with associated beta and gamma subunits. The alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their AMP-dependence and subcellular localization with alpha 2 complexes being partly nuclear. AMPK is activated allosterically by 5\' -AMP which also promotes phosphorylation and activation by an upstream kinase and inhibits dephosphorylation and inactivation. Elevation of AMP always accompanies depletion of ATP due to the action of adenylate kinase. Since high ATP antagonizes the activating effects of AMP the system behaves like a cellular \' fuel gauge\' . It is activated by various types of stress associated with ATP depletion such as hypoxia heat shock metabolic poisoning and in muscle exercise. AMPK phosphorylates multiple targets which switch off anabolic pathways and switch on alternative catabolic pathways.

ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium.

Heterotrimer of a catalytic subunit a beta and a gamma non-catalytic subunits.

Contains 1 protein kinase domain.