EGF

EGFNCBI

EGF

This product is specific for human EGF (Epidermal Growth Factor) a potent stimulator and regulator of the proliferation of epidermal and epithelial cells which is expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR) which also binds other EGF-like ligands with high affinity including TGF-? VGF (vaccinia virus growth factor) and Betacellulin produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways including the JAK/STAT and P13K/AKT pathways. **This product may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with as a detection reagent.

Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunohistology - Paraffin - 0. 25 - 1. 0ug/mlmmunohistology - Resin - 0. 5 - 2. 0ug/mlWestern Blotting - 0. 1 - 0. 2ug/mlFunctional Assays - 0. 25 - 0. 35ug/ml Summary: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. Summary: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. 1. Carpenter. G. and Cohen. S. (1990) Epidermal Growth Factor*. 2. Saito. T. et al. (2004) Differential activation of Epidermal Growth Factor (EGF) Receptor downstream signaling pathways by Betacellulin and EGF.