Phospho-Histone H3 (pS10)

GWB-D32D87

A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species based on sequence homology.

WB: 1:2 500IHC: 1:50-100ICC: 1:250IP: 1:40. Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin and is made up of four core histone proteins (H2A H2B H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9 14 18 and 23 (3 4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3 4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5). 1. Okada S Ohshima K Uehara Y et al Synip phosphorylation is required for insulin-stimulated Glut4 translocation Biochemical and Biophysical Research Communications 356 (2007) 102-106[Application: IP]2. Panomketa P Splitterc G Harmsc J et al TBK1 does not play a role in the control of in vitro Burkholderia pseudomallei growth Transactions of the Royal Society of Tropical Medicine and Hygiene 102/S1 (2008) S95 - S100[Application: WB]3. Ceder JA Jansson L Ehrnstrom RA et al The Characterization of Epithelial and Stromal Subsets of Candidate Stem/Progenitor Cells in the Human Adult Prostate European Urology 53 (2008) 524-532[Application: ICC]4. Lee ES Yoon CH Kim YS et al The double-strand RNA-dependent protein kinase PKR plays a significant role in a sustained ER stress-induced apoptosis FEBS Letters 581 (2007) 4325-4332[Application: WB]5. Ulbert S Antonin W Platani M et al The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology FEBS Letters 580 (2006) 6435-6441[Application: ICC]6. Fiskus W Wang Y Joshi R et al Cotreatment with Vorinostat Enhances Activity of MK-0457 (VX-680) against Acute and Chronic Myelogenous Leukemia Cells Clin Cancer Res. 14 (2008) 6106-6115[Application: WB]7. Angi M Damato B Taktak AFG et al Immunohistochemical Assesment of Mitotic Index in Uveal Melanoma Invest. Ophthalmol. Vis. Sci. 50 (2009) :6180[Application: IHC]8. Fiskus W Wang Y Joshi R et al Histone H3 (pS10) Clin Cancer Res. 14 (2008) 6106-6115[Application: WB]1. Braunstein M. et al. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol. Cell. Biol. 16: 43492. Workman J. L. and R. E. Kingston. Alteration of nucleosome structure as a mechanism of transcriptional regulation. Annu. Rev. Biochem. 67: 5453. Hansen J. C. et al. Structure and function of the core histone N-termini: more than meets the eye. Biochemistry 37 176374. Strahl B. D. and C. D. Allis. The language of covalent histone modifications. Nature 403 415. Hendzel M. J. et al. Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106: 3486. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487