PI3-kinase p110 subunit alpha

GWB-535B4A

A synthetic peptide of human near the C-term of PI3-K p110 alpha was used as an immunogen.

WB: 1:1 000-5 000ICC: 1:100-250IP: 1:30. PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found; and the subunit. The alpha isoform also known as PI3KCA is a transforming oncogene (3) that was shown to have activating mutations in nine types of cancers such as colon brain breast and stomach (4-5). Three hot spot mutations (E542K and E545K; and H1047R) make p110 a potential drug target. 1. Warrington NM Woerner BM Daginakatte GC et al Spatiotemporal Differences in CXCL12 Expression and Cyclic AMP Underlie the Unique Pattern of Optic Glioma Growth in Neurofibromatosis Type Cancer Res 67 (2007) 8588-8595[Application: WB]2. Nery FC Zeng J Niland BP et al TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton J Cell Sci 121 (2008) 3476-3486[Application: WB]3. Kim WY Chang DJ Hennessy B et al A Novel Derivative of the Natural Agent Deguelin for Cancer Chemoprevention and Therapy Cancer Prev Res 1 (2008) 577-587[Application: WB]4. Junttila TT Akita RW Parsons K et al Ligand-Independent HER2/HER3/PI3K Complex Is Disrupted by Trastuzumab and Is Effectively Inhibited by the PI3K Inhibitor GDC-0941 Cancer Cell 15 (2009) 429-440[Application: WB]5. Jaiswal BS Janakiraman V Kljavin NM et al PI3-kinase p110 subunit alpha Cancer Cell 16 (2009) 463-474[Application: WB]6. Levin VA Panchabhai SC Shen L et al Different Changes in Protein and Phosphoprotein Levels Result from Serum Starvation of High-Grade Glioma and Adenocarcinoma Cell Lines J Proteome Res 9 (2010) 179-191[Application: WB]1. Otsu et al. (1992) Cell 65 91-1042. Hiles et al. (1991) Cell 70 419-4293. Chang HW Aoki M Fruman D et al. Science 1997; 276:18484. Depowski PL Rosenthal SI Ross JS. Mod Pathol 2001; 14:6725. Bose S Crane A Hibshoosh H Mansukhani M Sandweis L Parsons R Hum Pathol 2002; 33:4056. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487