p73 delta N [38C674]

38C674

Synthetic peptide corresponding to amino acids 2-13 of Human p73 delta N.

This antibody does not cross react with full-length p73.

p73 delta N

Tris saline containing 0. 02% sodium azide and 0. 5% BSA

73 kDa. Cell extracts transfected with p73 delta N cDNA can be used as a positive control for this antibody. For optimal results primary antibody incubations should be performed at room temperature. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Even though the tumor suppressor gene p53 is highly important in human cancer as indicated by the fact that it is mutated in about 50% of cases up to a few years ago no similar proteins had been identified. Two p53 homologues are now identified p73 and p63 with high amino acid identity suggesting similar functions. Both p63 and p73 have two significant structural peculiarities compared with p53; the presence of an extended non-conserved C-terminus containing a sterile alpha motive (SAM) (typical of developmental proteins) and the presence of number of different splicing isoforms differing in the N-terminus or in the absence of the transactivation domain (delta N forms).

The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Isoform Beta interacts homotypically and with p53/TP53 whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma isoform Alpha and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Found in a complex with p53/TP53 and CABLES1. Isoform Beta interacts with WWOX. Interacts with HECW2.

Brain kidney placenta colon heart liver spleen skeletal muscle prostate thymus and pancreas.

Possesses an acidic transactivation domain a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.

Isoform alpha (but not isoform beta) is sumoylated on Lys-627 which potentiates proteasomal degradation but does not affect transcriptional activity.

Activated and stabilized by interaction with RANBP9.