COX1 [5F6/F4]

5F6/F4

A preparation of intracellular proteins of the human cell line HL60.

COX-1. No cross-reactivity with Cox-2.

Cytoplasmic

0. 02% Sodium Azide; Constituents: PBS (FCS free).

Cytoplasmic Cyclooxygenases (COX) are 72-kDa intralumenal residents of the endoplasmic reticulum (ER) and nuclear envelope where they catalyze the rate-limiting steps in the conversion of arachidonate to the physiologically dynamic prostanoids (Ballif et al. 1996). Numerous investigations have shown that the isoform COX-1 is not a participant in the pathway of inflammation process (Marnett 2000) and colon carcinogenesis (Prescott & Fitzpatrick 2000). Although research on COX-2 inhibitors has focused mainly on inflammation and pain experimental and epidemiological data suggest that NSAID (Non steroidal ant-inflammatory drugs) should be used without any effect on COX-1 in the treatment or prevention of a broader range of diseases (Marnett & Kalgutkar 1999).

Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O. Cofactor: Binds 1 heme B (iron-protoporphyrin IX) group per subunit (By similarity).

Homodimer.

This enzyme acts both as a dioxygenase and as a peroxidase.

Belongs to the prostaglandin G/H synthase family.