DNA polymerase beta [61]

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DNA polymerase beta protein

A 39 kDa protein known as DNA polymerase beta.

DNA polymerase beta

0. 1 mg/ml Storage

For IHC-P: Use at a dilution of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer pH 6. 0 prior to immunostaining. For WB: Use at an assay dependent diluiton. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. DNA polymerase beta comprises an amino-terminal 8 kD domain and a carboxy-terminal 31 kD domain. DNA Polymerase beta a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Cofactor: Binds 2 magnesium ions per subunit (By similarity).

Nucleus.

Belongs to the DNA polymerase type-X family.