Comparison

DNA polymerase beta [61]

Item no. 20-272-192241
Manufacturer GENWAY
Amount 0.25 ml
Category
Type Antibody
Applications WB, IHC
Clone 61
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GWB-C8D577
Similar products 20-272-192241
Available
Genway ID:
GWB-C8D577
Clone:
61
Isotype:
IgG2a
Immunogen:
DNA polymerase beta protein
Antigen Species:
N/A
Specificity:
A 39 kDa protein known as DNA polymerase beta.
Positive Control:
Testis
Target:
DNA polymerase beta
Localization:
Nuclear
Concentration:
0. 1 mg/ml Storage
Buffer:
Antibody solution containing sodium azide
Application Note:
For IHC-P: Use at a dilution of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer pH 6. 0 prior to immunostaining. For WB: Use at an assay dependent diluiton. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. DNA polymerase beta comprises an amino-terminal 8 kD domain and a carboxy-terminal 31 kD domain. DNA Polymerase beta a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
Function:
Repair polymerase. Conducts \" gap-filling\" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5\' -deoxyribose-5-phosphate lyase (dRP lyase) activity.
Catalytic Activity:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Cofactor: Binds 2 magnesium ions per subunit (By similarity).
Subunit:
Monomer.
Subcellular Location:
Nucleus.
Domain:
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity (By similarity).
Similarity:
Belongs to the DNA polymerase type-X family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.25 ml
Available: In stock
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