CD89

GWB-4584BD

A3Host Animal: Mouse. Hybridization of P3-X63-Ag. 8. 653

CD89 antigen (Fc alpha R) is a 50-75 kDa single chain transmembrane glycoprotein. -Fc alpha R can bind both monomeric and polymeric forms of IgA1 and IgA2 antibodies (1). The identified Fc alpha R gene encodes six potential N-glycosylation sites in the predicted extracellular region (2). The selective expression of Fc alpha R by myeloid lineage cells has been confirmed by analysis of Fc alpha R mRNA expression (2). A3 recognizes nonpolymorphic determinant located outside of the IgA-binding site of the Fc alpha R molecules (3). -The antibody stains more than 99% of peripheral blood granulocytes and 13+7% of the peripheral blood mononuclear cells (3). The majority of this subpopulation of blood mononuclear cells is CD15+. whereas CD3+ T cells. CD19+ B cells and CD57+ NK are non reactive (3). The bone marrow the antibody reacts with 50 - 75% of the myelomonocytic cells and granulocytes (3). The antibody does not block IgA binding.

0. 2mg/ml (prior to lyophilization)

2ug/5x105 cells/test. . Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. MAb to CD89. Monoclonal Antibody to CD89

Associates with the Fc epsilon RI gamma 2 receptor inducing tyrosine phosphorylation of gamma 2.

Isoform A. 2: Cell membrane; Single-pass type I membrane protein.

Isoform B: Secreted.

Differentially expressed between blood and mucosal myeloid cells. Monocytes express isoform A. 1 isoform A. 2 and isoform A. 3 while alveolar macrophages express isoform A. 1 and isoform A. 2; however they express only one isoform at their surfaces.