Leptin

IgG2a

Synthetic human leptin (131-145) attached to a carrier protein

Monoclonal Antibodies to Proteins and Bioactive Peptides

Not applicableApplications Notes : Has been found to stain specifically human adipocytes at a 1:1000 dilution. The ability to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 minutes with 4% normal goat serum reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated Goat anti-Mouse IgG. The immunofluorescent staining pattern was observed using epifluorescence microscopy. Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~16 kDa at a dilution of 1:4000. Suitable for use in IHC using frozen sections. Paraffin-embedded sections have not been tested. MAb to Leptin. Monoclonal Antibody to Human Leptin (residues 131-145)

Interacts with SIGLEC6.

Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].