Vimentin

IgG1

V9 reacts exclusively with vimentin. which is expressed in mesenchymal cells and mesenchymal derived tumors e. g. lymphoma. sarcoma and melanoma. V9 is suitable for immunoblotting. immunocytochemistry. immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6. 0) is required. Optimal antibody dilution should be determined by titration; recommended range is 1:25 ? 1:200 for flow cytometry. and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. and 1:100 ? 1:1000 for immunoblotting applications. Product

Vimentins are class-III intermediate filaments found in various non-epithelial cells especially mesenchymal cells.

Highly expressed in fibroblasts some expression in T- and B-lymphocytes and little or no expression in Burkitt\' s lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Belongs to the intermediate filament family. 1. Osborn. M. Debus. E. and Weber. K. (1984). Monoclonal antibodies specific for vimentin. Eur J Cell Biol 34. 137-43. 2. Tolle. H. G. Weber. K. and Osborn. M. (1985). Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments. Eur J Cell Biol 38. 234-44. 3. Van Muijen. G. N. Ruiter. D. J. and Warnaar. S. O. (1987). Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest 57. 359-69. 5. Corver. W. E. Koopman. L. A. van der Aa. J. Regensburg. M. Fleuren. G. J. and Cornelisse. C. J. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39. 96-107.