Hersteller |
GENWAY
|
Kategorie |
|
Typ |
Antibody |
Specific against |
other |
Clon |
5D8. 8. 12 |
Applikationen |
WB, FC, IHC |
Menge |
0.025 mg |
ArtNr |
20-783-310139 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Genway ID: |
GWB-6E2CC0 |
Specificity: |
CD162NCBI |
Gene ID: |
6404 |
Specificity: |
CD162 |
Clone: |
5D8. 8. 12 (PL2) |
Immunogen: |
Human neutrophils |
Fusion Partner: |
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63. Ag8. 653 myeloma cell line. |
Immunohistology: |
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6. 0 is recommended for this purpose. Histology: Tonsil |
Buffer Solution: |
Phosphate buffered saline |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide0. 1%Bovine Serum AlbuminSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Suggested |
Dilution: |
Flow Cytometry - 1/50Immunohistology - Frozen - 1/200 - 1/1000Immunohistology - Paraffin - 1/200 - 1/1000 |
Function: |
A SLe(x)-type glycan which through high affinity calcium-dependent interactions with E- P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. |
Subunit: |
Homodimer; disulfide-linked. Interaction with P- E- and L-selectins through their lectin/EGF domains is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20. |
Subcellular Location: |
Membrane; Single-pass type I membrane protein. |
Tissue Specificity: |
Expressed on neutrophils monocytes and most lymphocytes. |
Ptm: |
Displays complex core-2 sialylated and fucosylated O-linked oligosaccharides at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide Galbeta1--& gt; 4GlcNAcbeta1--& gt; 6(Galbeta1--& gt; 3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1 3 linkage present in two forms: One species is a disialylated monofucosylated glycan and the other a monosialylated trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation. |
Ptm: |
Sulfation in conjunction with the SLe(x)-containing glycan is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51 whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment and leukocyte rolling. 1. Moore. K. et al. (1995) P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. 2. Norman. K. E. et al. (1995) Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1. |
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