Antibodies

CD162

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone 5D8. 8. 12
Applications WB, FC, IHC
Amount 0.025 mg
ArtNr 20-783-310139
Available
Genway ID:
GWB-6E2CC0
Specificity:
CD162NCBI
Gene ID:
6404
Specificity:
CD162
Clone:
5D8. 8. 12 (PL2)
Immunogen:
Human neutrophils
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63. Ag8. 653 myeloma cell line.
Immunohistology:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6. 0 is recommended for this purpose. Histology: Tonsil
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium Azide0. 1%Bovine Serum AlbuminSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Suggested
Dilution:
Flow Cytometry - 1/50Immunohistology - Frozen - 1/200 - 1/1000Immunohistology - Paraffin - 1/200 - 1/1000
Function:
A SLe(x)-type glycan which through high affinity calcium-dependent interactions with E- P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.
Subunit:
Homodimer; disulfide-linked. Interaction with P- E- and L-selectins through their lectin/EGF domains is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Expressed on neutrophils monocytes and most lymphocytes.
Ptm:
Displays complex core-2 sialylated and fucosylated O-linked oligosaccharides at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide Galbeta1--& gt; 4GlcNAcbeta1--& gt; 6(Galbeta1--& gt; 3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1 3 linkage present in two forms: One species is a disialylated monofucosylated glycan and the other a monosialylated trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Ptm:
Sulfation in conjunction with the SLe(x)-containing glycan is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51 whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment and leukocyte rolling. 1. Moore. K. et al. (1995) P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. 2. Norman. K. E. et al. (1995) Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Price: €152.38
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