Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone 5D8. 8. 12
Applications WB, FC, IHC
Amount 0.025 mg
ArtNr 20-783-310139
Genway ID:
Gene ID:
5D8. 8. 12 (PL2)
Human neutrophils
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63. Ag8. 653 myeloma cell line.
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6. 0 is recommended for this purpose. Histology: Tonsil
Buffer Solution:
Phosphate buffered saline
Preservative Stabilisers:
0. 09%Sodium Azide0. 1%Bovine Serum AlbuminSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Suggested
Flow Cytometry - 1/50Immunohistology - Frozen - 1/200 - 1/1000Immunohistology - Paraffin - 1/200 - 1/1000
A SLe(x)-type glycan which through high affinity calcium-dependent interactions with E- P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.
Homodimer; disulfide-linked. Interaction with P- E- and L-selectins through their lectin/EGF domains is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Expressed on neutrophils monocytes and most lymphocytes.
Displays complex core-2 sialylated and fucosylated O-linked oligosaccharides at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide Galbeta1--& gt; 4GlcNAcbeta1--& gt; 6(Galbeta1--& gt; 3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1 3 linkage present in two forms: One species is a disialylated monofucosylated glycan and the other a monosialylated trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation in conjunction with the SLe(x)-containing glycan is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51 whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment and leukocyte rolling. 1. Moore. K. et al. (1995) P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. 2. Norman. K. E. et al. (1995) Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Price: €152.38

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Salesmanager: Arne Pelz