CD13

GWB-7CC18C

16790

R3-63

Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells macrophages granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

Homodimer. Interacts with the S1 domain of HCoV-229E spike protein.

Expressed in epithelial cells of the kidney intestine and respiratory tract; granulocytes monocytes fibroblasts endothelial cells cerebral pericytes at the blood-brain barrier synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).

N- and O-glycosylated.

Defects in ANPEP may be a cause of various types of leukemia or lymphoma.

Belongs to the peptidase M1 family [view classification]. 1. Hansen. A. S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibilty complex class II molecules. 2. Larsen. S . L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.