Hersteller |
GENWAY
|
Kategorie |
|
Typ |
Antibody |
Specific against |
other |
Clon |
53-6. 7 |
Applikationen |
FC, IP, IHC |
Menge |
0.025 mg |
ArtNr |
20-783-310648 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Genway ID: |
GWB-8CE70D |
Specificity: |
CD8 ALPHANCBI |
Gene ID: |
12525 |
Specificity: |
CD8 ALPHA |
Clone: |
53-6. 7 |
Immunogen: |
Mouse thymus or spleen. |
Immunohistology: |
This product has been reported as working well on Zinc-fixed paraffin-embedded sections. However. inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 2 |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide (NaN3) Suggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/). |
Function: |
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
Subunit: |
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. |
Subcellular Location: |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Subcellular Location: |
Isoform 2: Secreted. |
Ptm: |
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers while the four N-terminal cysteines do not (By similarity). |
Disease: |
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells leading to recurrent bacterial infections. |
Similarity: |
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Jabbari. A. and Harty. J. T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses. 2. Takahashi. K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells. |
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