Hersteller |
GENWAY
|
Kategorie |
|
Typ |
Antibody |
Specific against |
other |
Clon |
KT15 |
Applikationen |
FC, IHC |
Menge |
0.025 mg |
ArtNr |
20-783-310700 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Genway ID: |
GWB-A2421D |
Specificity: |
CD8 ALPHANCBI |
Gene ID: |
12525 |
Specificity: |
CD8 ALPHA |
Clone: |
KT15 |
Immunogen: |
T cell clone C6 |
Fusion Partner: |
Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 4 |
|
Preservative Stabilisers: |
| 0. 09%Sodium AzideSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested |
Dilution: |
Flow Cytometry - 1/50 - 1/100 |
Function: |
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
Subunit: |
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. |
Subcellular Location: |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Subcellular Location: |
Isoform 2: Secreted. |
Ptm: |
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers while the four N-terminal cysteines do not (By similarity). |
Disease: |
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells leading to recurrent bacterial infections. |
Similarity: |
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Tomonari. K. et al. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11 positive mouse T-cell clone. 2. Whiteland. J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.