Hersteller |
GENWAY
|
Kategorie |
|
Typ |
Antibody |
Specific against |
other |
Clon |
M1/70. 15 |
Applikationen |
FC |
Menge |
25 TESTS |
ArtNr |
20-783-310716 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Genway ID: |
GWB-3B9D1F |
Specificity: |
CD11bNCBI |
Gene ID: |
16409 |
Specificity: |
CD11b |
Clone: |
M1/70. 15 |
Immunogen: |
T cells enriched splenocytes from B10 mice. |
Fusion Partner: |
Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1. Ag4. 1 myeloma cell line. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 4 |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide1%Bovine Serum Albumin Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/). Suggested |
Dilution: |
Flow Cytometry - Neat - 1/10 |
Function: |
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3 the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. |
Subunit: |
Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. |
Subcellular Location: |
Membrane; Single-pass type I membrane protein. |
Tissue Specificity: |
Predominantly expressed in monocytes and granulocytes. |
Domain: |
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. |
Disease: |
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin joints kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. |
Similarity: |
Belongs to the integrin alpha chain family. |
Similarity: |
Contains 7 FG-GAP repeats. |
Similarity: |
Contains 1 VWFA domain. 1. Springer. T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. 2. Springer. T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by a monoclonal antibody. 3. Beller. D. I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor 4. Whiteland. J. L. et al. (1995) Immunohistochemical detection of T cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. 5. Klingel. K. et al. (2003) Beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis. |
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