Toxoplasma IgG Enzyme Immunoassay Test Kit

IGG Antibodies to Toxoplasma gondiiSample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Biotech Toxoplasma IgG ELISA is intended for use in evaluating serologic status to Toxoplasma gondii infection. Introduction: Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by consuming contaminated meat or by coming in contact with cat feces containing oocysts. In adolescence and adulthood most infections are subclinical. However if a pregnant woman contracts toxoplasmosis it may be passed through the placenta to the fetus resulting in congenital toxoplasmosis which is a cause of mortality and malformation. Asymptomatic infants may develop abnormalities later in life. Principle of the test: Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted serum is added to the wells and the Toxoplasma gondii IgG-specific antibody if present binds to the antigen. All unbound materials are washed away. HRP-conjugate is added which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understandingof the package insert instructions and with adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. Precision: A study was performed to document typical assay precision with the Biotech Toxo IgG EIA product. The mean SD and %CV were calculated for Intra- and Inter-Assay in concentration value (IU/mL).

Secreted.

Nie has the G1M(17) allotypic marker 97-K and the G1M(1) markers 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

EU also differs in the amidation states of residues 155 166 177 195 198 269 and 272 and in the order of residues 268-272.