CALR Antibody (Center)

Synthetic Peptide

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Polyclonal

811

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-CALR Antibody (Center) at 1:2000 dilution + PC-3 whole cell lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 55 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

All lanes : Anti-CALR Antibody (Center) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: SH-SY5Y whole cell lysate
Lane 5: Ramos whole cell lysate
Lane 6: A549 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 48 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

All lanes : Anti-CALR Antibody (Center) at 1:1000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Ramos whole cell lysate
Lane 6: SH-SY5Y whole cell lysate
Lane 7: PC-3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 48 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

Overlay histogram showing HeLa cells stained with AP6898c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6898c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

Pr

48142

HUMAN

NP_004334.1