LIF Antibody (Center)

P15018

WB, FC, IHC-P-Leica

Novotny, Z., et.al., Folia Biol. (Praha) 55 (3), 92-97 (2009)

RB21725

LIF

H, Rat

WB

All lanes : Anti- LIF Antibody (Center) at 1:1000 dilution
Lane 1: KG-1 whole cell lysate
Lane 2:Human spleen whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 22 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P-Leica

Overlay histogram showing U-2 OS cells stained with AP6981C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6981C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

22008

HUMAN

NP_001244064.1; NP_002300.1