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LIF Antibody (Center)

LIF Antibody (Center)

Item no.	ABC-AP6981C
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	LIF, HILDA, D factor, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, Emfilermin
Available
Primary Accession	P15018
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P-Leica
Bio Background	LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Bio References	Novotny, Z., et.al., Folia Biol. (Praha) 55 (3), 92-97 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB21725
Gene ID	3976
Gene Name	LIF
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, Rat
Legend image 1	Anti-LIF Antibody (Center) at 1:1000 dilution + Rat spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti- LIF Antibody (Center) at 1:1000 dilution Lane 1: KG-1 whole cell lysate Lane 2:Human spleen whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP6981C performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P-Leica
Dilution image 3	1:500
Legend image 4	Overlay histogram showing U-2 OS cells stained with AP6981C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6981C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 4	FC
Dilution image 4	1:25
Isotype	Rabbit Ig
Calculated MW	22008
Antigen Region	72-101
Antigen Source	HUMAN
Target/Specificity	This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human LIF.
NCBI Accession	NP_001244064.1; NP_002300.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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