PKA C-beta (PRKACB) Antibody (N-term)

Synthetic Peptide

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.

Polyclonal

5567

Purified Rabbit Polyclonal Antibody (Pab)

Anti-PRKACB Antibody (K29) at 1:1000 dilution + human brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 41 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

WB

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

1:50~100

40623

HUMAN

NP_001229786.1; NP_001229787.1; NP_001229788.1; NP_001229789.1; NP_001229790.1; NP_001229791.1; NP_002722.1; NP_891993.1; NP_997461.1