IDH1 Antibody (Center)

Synthetic Peptide

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Polyclonal

3417

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-IDH1 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution.

Observed band size : 47kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IF

Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)(Cat. #AP7454c). AP7454c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

1:1000

WB

Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)(Cat#AP7454c). AP7454c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

1:100

IHC-P

IDH1 Antibody (Center) (Cat. #AP7454c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

B, Rat, Sh

46659

HUMAN

NP_001269315.1; NP_001269316.1; NP_005887.2