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IDH1 Antibody (Center)

IDH1 Antibody (Center)

Item no.	ABC-AP7454c
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IDH1, IDH, IDP, PICD, Cytosolic NADP-isocitrate dehydrogenase, Isocitrate dehydrogenase [NADP] cytoplasmic, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH
Available
Primary Accession	O75874
Antigen Type	Synthetic Peptide
Application	WB, IHC-P, FC, IF
Bio Background	IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Bio References	Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999) Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004) Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009)
Clonality	Polyclonal
Clone/Animal Names	RB18238
Gene ID	3417
Gene Name	IDH1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-IDH1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 47kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AP7454c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 2	IF
Dilution image 2	1:10~50
Legend image 3	Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)(Cat. #AP7454c). AP7454c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of IDH1 Antibody (Center) (Cat.#AP7454c) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)(Cat#AP7454c). AP7454c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 5	IHC-P
Dilution image 5	1:100
Legend image 6	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 6	IHC-P
Dilution image 6	1:50~100
Legend image 7	IDH1 Antibody (Center) (Cat. #AP7454c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 7	FC
Dilution image 7	1:10~50
Other Accession	P41562; O88844; Q9XSG3; Q6XUZ5
Predicted	B, Rat, Sh
Isotype	Rabbit Ig
Calculated MW	46659
Antigen Region	116-143
Antigen Source	HUMAN
Target/Specificity	This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
NCBI Accession	NP_001269315.1; NP_001269316.1; NP_005887.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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