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VEGFR2 (FLK1/KDR) Antibody (C-term)

VEGFR2 (FLK1/KDR) Antibody (C-term)

ArtNr	ABC-AP7643a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	VEGFR2, KDR, Vascular endothelial growth factor receptor 2, Fetal liver kinase 1, Protein-tyrosine kinase receptor flk-1, FLK1, Kinase insert domain receptor, VEGFR-2, CD309, FLK-1
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Primary Accession	P35968
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, IF, WB
Bio Background	KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Bio References	Yilmaz, A., et al., Biochem. Biophys. Res. Commun. 306(3):730-736 (2003). Zeng, H., et al., J. Biol. Chem. 278(23):20738-20745 (2003). Kiba, A., et al., J. Biol. Chem. 278(15):13453-13461 (2003). Elvert, G., et al., J. Biol. Chem. 278(9):7520-7530 (2003). Yang, S., et al., Arterioscler. Thromb. Vasc. Biol. 22(11):1797-1803 (2002).
Clonality	Polyclonal
Clone/Animal Names	RB01536
Gene ID	3791
Gene Name	KDR (HGNC:6307)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term)(Cat#AP7643a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of anti-KDR/FLK1 Pab (Cat. #AP7643a) in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	VEGFR2 (FLK1/KDR) Antibody (C-term) (Cat. #AP7643a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	151527
Antigen Region	1326-1356
Antigen Source	HUMAN
Target/Specificity	This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1356 amino acids from the C-terminal region of human VEGFR2 (FLK1/KDR).
NCBI Accession	NP_002244.1

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Menge: 400 ul

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