Comparison

VEGFR2 (FLK1/KDR) Antibody (C-term)

Item no. ABC-AP7643a
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications WB, FC, IF, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products VEGFR2, KDR, Vascular endothelial growth factor receptor 2, Fetal liver kinase 1, Protein-tyrosine kinase receptor flk-1, FLK1, Kinase insert domain receptor, VEGFR-2, CD309, FLK-1
Available
Primary Accession
P35968
Antigen Type
Synthetic Peptide
Application
IHC-P, FC, IF, WB
Bio Background
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Bio References
Yilmaz, A., et al., Biochem. Biophys. Res. Commun. 306(3):730-736 (2003).
Zeng, H., et al., J. Biol. Chem. 278(23):20738-20745 (2003).
Kiba, A., et al., J. Biol. Chem. 278(15):13453-13461 (2003).
Elvert, G., et al., J. Biol. Chem. 278(9):7520-7530 (2003).
Yang, S., et al., Arterioscler. Thromb. Vasc. Biol. 22(11):1797-1803 (2002).
Clonality
Polyclonal
Clone/Animal Names
RB01536
Gene ID
3791
Gene Name
KDR (HGNC:6307)
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term)(Cat#AP7643a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
Western blot analysis of anti-KDR/FLK1 Pab (Cat. #AP7643a) in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3
IHC-P
Dilution image 3
1:50~100
Legend image 4
VEGFR2 (FLK1/KDR) Antibody (C-term) (Cat. #AP7643a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Isotype
Rabbit Ig
Calculated MW
151527
Antigen Region
1326-1356
Antigen Source
HUMAN
Target/Specificity
This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1356 amino acids from the C-terminal region of human VEGFR2 (FLK1/KDR).
NCBI Accession
NP_002244.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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