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SRC Antibody (N-term)

SRC Antibody (N-term)

ArtNr	ABC-AP7718a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SRC, SRC1, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src
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Primary Accession	P12931
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P
Bio Background	The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.
Bio References	Lopez, R.G., et al., J. Biol. Chem. 278(42):41316-41325 (2003). Horn, S., et al., Oncogene 22(46):7170-7180 (2003). Hakam, A., et al., Dig. Dis. Sci. 48(10):1972-1978 (2003). Ito, H., et al., Surgery 134(2):221-226 (2003). Bouchard, M.J., et al., J. Virol. 77(14):7713-7719 (2003).
Clonality	Polyclonal
Clone/Animal Names	RB3093
Gene ID	6714
Gene Name	SRC (HGNC:11283)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Confocal immunofluorescent analysis of SRC Antibody (N-term)(Cat#AP7718a) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of lysates from MCF-7, rat PC-12, mouse C2C12 cell line (from left to right), using SRC Antibody (R14)(Cat. #AP7718a).AP7718a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Isotype	Rabbit Ig
Calculated MW	59835
Antigen Region	1-30
Antigen Source	HUMAN
Target/Specificity	This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SRC.
NCBI Accession	NP_005408.1; NP_938033.1

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ABC-AP7718a-datasheet.pdf

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Menge: 400 ul

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