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PRKCA Antibody (N-term)

PRKCA Antibody (N-term)

ArtNr	ABC-AP8576a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PRKACA, PRKCA, Protein kinase C alpha type, PKCA, PKC-alpha, PKC-A
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Primary Accession	P17252
Antigen Type	Synthetic Peptide
Application	IF, FC, IHC-P, WB
Bio Background	PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Bio References	Gysin, S. and Imber, R. et.al., Eur. J. Biochem. 240 (3), 747-750 (1996) Rider, M.H., et.al., Biochem. J. 285 (PT 2), 405-411 (1992)
Clonality	Polyclonal
Clone/Animal Names	RB20769
Gene ID	5578
Gene Name	PRKCA
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Confocal immunofluorescent analysis of PRKCA Antibody (N-term)(Cat#AP8576a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of PRKCA Antibody (N-term) (Cat. #AP8576a) in mouse NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	PRKCA Antibody (N-term) (Cat. #AP8576a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P05696; P10102; P20444; P04409
Predicted	B, Rb, Rat
Isotype	Rabbit Ig
Calculated MW	76750
Antigen Region	75-103
Antigen Source	HUMAN
Target/Specificity	This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA.
NCBI Accession	NP_002728.1

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Menge: 400 ul

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