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PRKCA Antibody (N-term)

PRKCA Antibody (N-term)

Item no.	ABC-AP8576a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PRKACA, PRKCA, Protein kinase C alpha type, PKCA, PKC-alpha, PKC-A
Available
Primary Accession	P17252
Antigen Type	Synthetic Peptide
Application	IF, FC, IHC-P, WB
Bio Background	PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Bio References	Gysin, S. and Imber, R. et.al., Eur. J. Biochem. 240 (3), 747-750 (1996) Rider, M.H., et.al., Biochem. J. 285 (PT 2), 405-411 (1992)
Clonality	Polyclonal
Clone/Animal Names	RB20769
Gene ID	5578
Gene Name	PRKCA
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Confocal immunofluorescent analysis of PRKCA Antibody (N-term)(Cat#AP8576a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of PRKCA Antibody (N-term) (Cat. #AP8576a) in mouse NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	PRKCA Antibody (N-term) (Cat. #AP8576a) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P05696; P10102; P20444; P04409
Predicted	B, Rb, Rat
Isotype	Rabbit Ig
Calculated MW	76750
Antigen Region	75-103
Antigen Source	HUMAN
Target/Specificity	This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA.
NCBI Accession	NP_002728.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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