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IGFBP2 Antibody (C-term)

IGFBP2 Antibody (C-term)

ArtNr	ABC-AP8588B
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IGFBP-2, IGFBP2, IBP2, IBP-2, Insulin-like growth factor-binding protein 2, IGF-binding protein 2, BP2
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Primary Accession	P18065
Antigen Type	Synthetic Peptide
Application	FC, IF, IHC-P, WB
Bio Background	IGFBP2-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Bio References	Arafat, A.M., et.al., J. Clin. Endocrinol. Metab. 94 (12), 5093-5101 (2009) Yazawa, T., et.al., Am. J. Pathol. 175 (3), 976-987 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB22493
Gene ID	3485
Gene Name	IGFBP2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of IGFBP2 Antibody (C-term)(Cat. #AP8588b) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Anti-IGFBP2 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P24853; P13384; Q29400
Predicted	B, Pig, Sh
Isotype	Rabbit Ig
Calculated MW	34814
Antigen Region	277-305
Antigen Source	HUMAN
Target/Specificity	This IGFBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of human IGFBP2.
NCBI Accession	NP_000588.2

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ABC-AP8588B-datasheet.pdf

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Menge: 400 ul

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