SRC Antibody (Y419)

ArtNr	ABC-AP8596A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SRC, SRC1, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src
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Primary Accession	P12931
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P
Bio Background	SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Bio References	Muller, A.J., et.al., Mol. Cell. Biol. 12 (11), 5087-5093 (1992) MacAuley, A., et.al., Oncogene 8 (1), 117-124 (1993) den Hertog, J., et.al., EMBO J. 12 (10), 3789-3798 (1993)
Clonality	Polyclonal
Clone/Animal Names	RB21492
Gene ID	6714
Gene Name	SRC (HGNC:11283)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of SRC Antibody (Y419)(Cat#AP8596a) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of SRC Antibody (Y419) (Cat. #AP8596a) in HepG2 cell line lysates (35ug/lane). SRC (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419)(Cat. #AP8596a). AP8596a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human hepatocarcinoma with SRC Antibody (Y419), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Other Accession	Q02977; P10936; F1LM93; Q04736; P07947; A1A5H8; P09324; Q9WUD9; P05480; Q1JPZ3; P00523; P13116; P13115; P13406; Q62844; A1Y2K1; P39688; P06241; Q05876; A0JNB0; F1RDG9; Q6EWH2
Predicted	Zf, B, C, M, Pig, Rat, X
Isotype	Rabbit Ig
Calculated MW	59835
Antigen Region	397-424
Antigen Source	HUMAN
Target/Specificity	This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
NCBI Accession	NP_005408.1; NP_938033.1

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