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SRC Antibody (Y419)

SRC Antibody (Y419)

Item no.	ABC-AP8596A
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SRC, SRC1, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src
Available
Primary Accession	P12931
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P
Bio Background	SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Bio References	Muller, A.J., et.al., Mol. Cell. Biol. 12 (11), 5087-5093 (1992) MacAuley, A., et.al., Oncogene 8 (1), 117-124 (1993) den Hertog, J., et.al., EMBO J. 12 (10), 3789-3798 (1993)
Clonality	Polyclonal
Clone/Animal Names	RB21492
Gene ID	6714
Gene Name	SRC (HGNC:11283)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of SRC Antibody (Y419)(Cat#AP8596a) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of SRC Antibody (Y419) (Cat. #AP8596a) in HepG2 cell line lysates (35ug/lane). SRC (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419)(Cat. #AP8596a). AP8596a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human hepatocarcinoma with SRC Antibody (Y419), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Other Accession	Q02977; P10936; F1LM93; Q04736; P07947; A1A5H8; P09324; Q9WUD9; P05480; Q1JPZ3; P00523; P13116; P13115; P13406; Q62844; A1Y2K1; P39688; P06241; Q05876; A0JNB0; F1RDG9; Q6EWH2
Predicted	Zf, B, C, M, Pig, Rat, X
Isotype	Rabbit Ig
Calculated MW	59835
Antigen Region	397-424
Antigen Source	HUMAN
Target/Specificity	This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
NCBI Accession	NP_005408.1; NP_938033.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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