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GAPDHS Antibody (Center)

GAPDHS Antibody (Center)

ArtNr	ABC-AP8610c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GAPDH-2, GAPDHS, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, GAPD2, GAPDS, GAPDH2, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
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Primary Accession	O14556
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	GAPDHS is a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1, 3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
Bio References	Welch, J.E., et.al., J. Androl. 21 (2), 328-338 (2000) Goodwin, L.O., et.al., Mol. Hum. Reprod. 6 (2), 127-136 (2000) Benham, F.J. et.al., Genomics 5 (2), 209-214 (1989)
Clonality	Polyclonal
Clone/Animal Names	RB16542
Gene ID	26330
Gene Name	GAPDHS
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of GAPDHS Antibody (Center) (Cat. #AP8610c) in NCI-H460 cell line lysates (35ug/lane). GAPDHS (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	GAPDHS Antibody (Center) (Cat. #AP8610c) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAPDHS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	GAPDHS Antibody (Center) (Cat. #AP8610c) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	Q4R3T1
Predicted	Pr
Isotype	Rabbit Ig
Calculated MW	44501
Antigen Region	104-134
Antigen Source	HUMAN
Target/Specificity	This GAPDHS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human GAPDHS.
NCBI Accession	NP_055179.1

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Menge: 400 ul

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