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SFRP1 Antibody (N-term)

SFRP1 Antibody (N-term)

ArtNr	ABC-AP9037A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	sFRP-1, SFRP1, FRP1, FRP, FRP-1, SARP2, SARP-2, Secreted frizzled-related protein 1, Secreted apoptosis-related protein 2
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Primary Accession	Q8N474
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	SFRP1 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer.
Bio References	Huang, D., et.al., J. Cancer Res. Clin. Oncol. 136 (3), 395-401 (2010); Yang, Z.Q., et.al., Int. J. Cancer 125 (7), 1613-1621 (2009).
Clonality	Polyclonal
Clone/Animal Names	RB21557
Gene ID	6422
Gene Name	SFRP1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of SFRP1 Antibody (N-term) (Cat. #AP9037a) in K562 cell line lysates (35ug/lane). SFRP1 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human testis tissue reacted with SFRP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Legend image 3	SFRP1 Antibody (N-term) (Cat. #AP9037a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Isotype	Rabbit Ig
Calculated MW	35386
Antigen Region	26-55
Antigen Source	HUMAN
Target/Specificity	This SFRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human SFRP1.
NCBI Accession	NP_003003.3

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