Gemcitabine HCl

ArtNr	S1149-1000
Hersteller	Selleckchem
CAS-Nr.	122111-03-9
Menge	1 g
Quantity options	100 mg 1 g 10 g 10 mM/1 ml 25 mg 300 mg 5 mg 5 g
Kategorie	Oncology Inhibitors & Compound Libraries Small Molecules By Organ Pancreatic Cancer
Typ	Inhibitors
Specific against	other
Smiles	C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl
ECLASS 10.1	32160490
ECLASS 11.0	32160490
UNSPSC	12000000
Alias	NSC 613327 HCl,LY-188011 HCl
Similar products	Gemcitabine
Lieferbar
Storage Conditions	2 years -80 in solvent
Molecular Weight	299, 66
Administration	Administered via i.p.
Animal Models	Athymic nude mice with MIA PaCa-2 cells
Cell lines	BxPC-3, MIA PaCa-2, and PANC-1 cells
Clinical Trials	Gemcitabine plus PEGPH20 has entered in a phase II clinical trial in the treatmentof the stage IV pancreatic cancer.
Concentrations	0.2 uM
Dosages	50 mg/kg or 100 mg/kg
Formulation	Phosphate-buffered saline
IC50	50 nM, 50 nM, 50 nM, 50 nM, 50 nM, 50 nM
In vitro	Gemcitabine induced NF-kappaB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-kappaB inhibitor IkappaBalpha in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-kappaB binding. In contrast, NF-kappaB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h, however, 24-h treatment with these higher doses increases NF-kappaB binding in BxPC-3 cells [2]
In vivo	Intratumoral NF-kappaB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-kappaB activation. [2]
Incubation Time	24 hours or 48 hours
Method	BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
Solubility (25C)	DMSO <1 mg/mL, Water 23 mg/mL, Ethanol <1 mg/mL
Information	Gemcitabine HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Chemical Name	4-amino-1-((2R, 4R, 5R)-3, 3-difluoro-4-hydroxy-5-(hydroxymethyl)-tetrahydrofuran-2-yl)pyrimidin-2(1H)-one hydrochloride
Features	Gemcitabine has been used for pancreatic cancer as the most effective anticancer drug.

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