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CheKine™ Micro Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit

ArtNr ABK-KTB1122-96T
Hersteller Abbkine Scientific
Menge 96 T
Quantity options 48 T 96 T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Phosphopyruvate carboxylase,Phosphoenolpyruvic carboxylase,PHOSPHOENOLPYRUVATE CARBOXYLASE,Phosphoenolpyruvic acid cocarboxylase,Phospho(enol)pyruvate Carboxylase corn,Carboxylase,phosphopyruvate (phosphate)
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Cell metabolism detection kit
Manufacturer - Applications
The kit provides a simple method for detecting Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial. In the assay, Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The rate of NADH decrease at 340 nm can reflect Phosphoenolpyruvate carboxylase (PEPC) activity.
Manufacturer - Category
Cell analysis
Manufacturer - Conjugate / Tag
PEPC
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Background
Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) is widely present in animals, plants, microorganisms and cells. It is an enzyme that catalyzes the reaction of phosphoenol acetic acid with gas phase to produce oxaloacetic acid and presents an irreversible reaction. The operation of the acid cycle plays an important regulatory role.
Description
Abbkine CheKine™ Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit is designed for the quantitative determination of PEPC activity.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Determination of Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage Notes
• Do not mix or substitute reagents or materials from other kit lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
• Avoid foaming or bubbles when mixing or reconstituting components.
• To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
• Ensure all reagents and solutions are at the appropriate temperature before starting the assay.
Kit components
• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III Stock solution
• Reagent III Buffer
Manufacturer - Search Terms
Phosphopyruvate carboxylase, Phosphoenolpyruvic carboxylase, PHOSPHOENOLPYRUVATE CARBOXYLASE, Phosphoenolpyruvic acid cocarboxylase, Phospho(enol)pyruvate Carboxylase corn, Carboxylase, phosphopyruvate (phosphate)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T
Lieferbar: In stock
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