ReverTra Ace qPCR RT Kit

ReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase " ReverTra Ace™ " and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min.
ReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
ReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase " ReverTra Ace™ " , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.

Features

• The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
• The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
• Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.