LeaBiozyme® Salt active ultraNuclease, Tag free

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.If the package is opened and stored at 4°C for more than one week, it is recommended to sterilize the product using a filter to prevent microbial contamination.

Salt Active ultraNuclease LeaBiozyme® is a recombinant nonspecific endonuclease expressed in Escherichia coli (E. coli). Compared to Benzonase that can only be used in salt-free or low-salt buffers, this product exhibits maximum activity in solutions containing 0.7 M NaCl and retains over 30% activity in solutions containing 0.9 M NaCl. It is provided as an enzyme solution in the storage buffer (25 mM Tris-HCl, 5 mM MgCl2, 500 mM NaCl, pH 7.5).We have directional protein refolding technology (LeaBioFOLD) and self-developed technology platforms for proteins covering screening and purification, engineering design, fixed-point coupling design, and dosage form development.Protein refolding is a process of recovering protein aggregates in inclusion bodies in the form of misfolded and inactive proteins expressed by prokaryotes such as Escherichia coli back into proteins with correct conformation and bioactivity under appropriate conditions in vitro. It is a key technology for biopharmaceutical companies but a major bottleneck in protein production in prokaryotic expression systems. Leading Biology has been specializing in protein refolding for many years, has a core team with over ten years of experience in this technology and rich experience in its industrialization. Our team is summarizing the experience to form an independent technological system of ours.

< 1.0 EU per μg of the protein as determined by the LAL method.

Salt active nuclease

250-300 U/μLWorking Temperature: 4~42°C, optimal activity at 25°C.Cofactor: 1-10 mM Mg2+ Unit of Definition: One unit (U) is defined as the amount of enzyme that cause a ΔA260 of 1.0 under conditions of excess substrate at 37°C for 30 minutes.