Anti-Mouse CD29 (Clone KMI6) - Biotin - 500 µg

ELISA, EM, FA, FC, IF Microscopy, IP, WB

2 - 8°C Wet Ice

Stromal cells are needed for the early steps of B lymphocyte differentiation and proliferation1.CD29, also known as integrin beta 12, is a 110 kDa protein discovered due to its expression onbone marrow stromal cells, at cell surface areas that interact with undifferentiated lymphoid cells1. Integrin beta 1 is a member of a family of alpha beta heterodimers that act as cell surfacereceptors for extracellular matrix proteins3, including collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others4. CD29 is also involved in sperm-egg fusion. Throughthese interactions, CD29 regulates and/or promotes cell adhesion, laminin matrix deposition, endothelial cell motility, angiogenesis, osteoblast compaction, the formation of mineralized bonenodules, and cytokinesis.KMI6 was raised against the B cell-supportive stromal cell line BMS21. LOU/MN rats wereimmunized with BMS2 cells and subsequently spleen cells were fused with murine Sp2/0 forhybridoma generation. Antibody recognition is insensitive to the glycosylation state but is lostwhen the CD29 protein is reduced for Western blotting2. As such, the epitope is likelyconformation-dependent. KMI6 was used to identify CD292. KMI6 can enhance cell adhesion offibronectin to CD4 -8- thymocytes3.

0.5 mg/ml

CD29 is present on the plasma membranes of many stromal cells, bonemarrow stromal cell line BMS2 in vitro, and a small number of cells in bone marrow cellsuspensions from adult mice. CD29 is also expressed in the media layer of the arterial wall.CD29 is expressed in skeletal and cardiac muscles. CD29 is also constitutively expressed bysome immature thymocytes. CD29 can be slightly induced on mature thymocytes and peripheralT cells by activation with Con A.