Anti-Mouse CD107b (LAMP-2/Mac-3) (Clone M3/84) - FITC - 50 µg

FC, ICC, IHC, IP, RIA

2 - 8°C Wet Ice

Macrophages function in phagocytosis of foreign organisms and materials as well as resistance totumors1. Additionally, they play roles in T lymphocyte immune responsiveness, inflammatoryresponses, and regulation of hematopoiesis. Lysosomes are the membrane-bound vacuoles thatdigest foreign materials and perform specialized autolytic functions2. Lysosomes also play a rolein antigen presentation, cholesterol metabolism, and hydrolysis of exogenous materials3 . CD107b (LAMP-2, Mac-3) is a member of the lysosome-associated membrane protein (LAMP)family and is an important component of the lysosomal membrane involved in autophagy3, 4 .CD107b is a highly glycosylated transmembrane protein, with glycosylation being necessary forits stability5, 6. Mice deficient in CD107b have a severe phenotype, with 50% dying aged 20-40days6. In humans, mutations in CD107b lead to Danon disease. The immunogen used to generate M3/84 was produced by first purifying surface glycoproteinsfrom macrophage membranes of peritoneal exudate cells of C57BL/6 mice5. Next, previouslyrecognized antigens were removed by immunoadsorbent columns to obtain a glycoprotein-enriched fraction. Then, (Lewis x BN) F1 rats were injected and splenocytes fused to mousemyeloma NS1 for hybridoma production.

0.2 mg/ml

CD107b (LAMP-2) is a constituent of the lysosomal membrane. CD107bis present in macrophages, granulocytes, peripheral blood lymphocytes, bronchial epithelium, alveolar lining cells, intestinal epithelium, and some dendritic and endothelium cells.