Anti-Human CXCR2 (Clone 4D1.5.7) - Purified No Carrier Protein - 100 µg

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2 - 8°C Wet Ice

Chemokines (chemoattractant cytokines) play an important role in immune and cancer cellmigration 1, metastasis, leukocyte trafficking to inflammatory sites 2, angiogenesis, neutrophilchemotaxis and activation 3 as well as cutaneous burn wound repair. At least 20 chemokinereceptors have been identified, among them CXCR2 (IL-8RB). CXCR2 is an interleukin (IL)-8(CXCL8) receptor that also binds to the CXC chemokines CXCL1 (GROα), CXCL2, CXCL3, CXCL4, CXCL5 (ENA78), CXCL6, and CXCL7 (NAP-2) 1. CXCR2 is also activated by MIF(macrophage migration inhibitory factor). Ligand binding occurs via the extracellular N-terminus. CXCR2 can exist as a homodimer or heterodimer with CXCR1 1. Many cancers thathave poorer patient prognosis are associated with increased CXCR2 expression.4D1.5.7 (also known as HB-11495) was generated by immunizing mice with 293 cells thatexpress CXCR2 4. Hybridomas were selected based on their ability to bind human neutrophils. Inflow cytometry assays, 4D1.5.7 bound to cells expressing CXCR2 but not to cells expressingCXCR1. ELISA experiments were subsequently used to identify residues 1-18 of the N-terminalas containing the binding site. Subsequent epitope mapping concluded that 4D1.5.7 recognizesresidues 6-10 (FEDFW) of CXCR2 as its binding epitope 2. 4D1.5.7 can inhibit IL-8 binding tocells expressing CXCR2 by up to 90% and to human neutrophils by up to 50% 4.

≥1.0 mg/ml

CXCR2 is expressed on neutrophils, monocytes, subsets of T lymphocytes, endothelial cells, and keratinocytes. CXCR2 is also expressed in brain tissues, particularlyneurons in the normal brain. Additionally, dystrophic neurites associated with some senileplaques strongly express CXCR2.