Anti-Human CD45 (Clone 2D1) - Dylight® 650 - 25 µg

Primary Monoclonal Antibodies>SurfaceTag Human CD Markers

2 - 8°C Wet Ice

CD45 is a receptor protein tyrosine phosphatase involved in immune cell activation1. It is a largeglycoprotein (180-220 kDa) with a broad range of expression in the animal kingdom, includingmammals, chicken, shark, and mosquitoes. The cytoplasmic region of CD45 is highly conservedin mammals but its extracellular region varies between species. Additionally, differential splicingleads to several CD45 isoforms which can vary in glycosylation, which in turn can affect CD45ligand interaction and function. In T cells, isoform expression is dependent on the stage of T cellmaturation, activation, and differentiation.CD45 functions in the immunological synapse of the T cell-antigen presenting cell contact zonethat is formed when T cells encounter cognate antigens presented by major histocompatibility(MHC) molecules 1. CD45 interacts with Src kinases, in particular Lck, to mediate antigenreceptor signaling in T and B cells, and is required for the development and activation oflymphocytes. CD45 also interacts with CD3ζ and CD3ε chains as well as tyrosine kinase Zap 70, DAP12, the Janus kinase family, PAG, CD22, among others. With these interactions, CD45functions to protect the host against viral, bacterial, and fungal infections.Certain variants of CD45 are associated with autoimmune disease and dysregulation is observedin some cancers 1. CD45 has been explored as a target of therapeutic modulation for organtransplantation 1, 2 , autoimmune disease 1, bone marrow transfusion 3, 4, 5, 6 as well as cancerradioimmunotherapy 7, 8, including a bispecific antibody targeting CD45 and 90 Y-DOTA fortherapy of myeloid leukemia 9.2D1 was generated by immunizing BALB/c mice with human peripheral blood mononuclearcells 10, 11. Spleen cells were fused to the P3/NSI/I-Ag4-1 myeloma line and resulting supernatantsscreened by an indirect I125 labeled antiglobulin binding assay.CD45 is also known as leukocyte common antigen (LCA), lymphocyte antigen 5 (Ly-5), andT200 12. 2D1 is also known as anti-HLe-1 10, 11.

0.2 mg/ml

CD45 is expressed on the surface of all nucleated hematopoietic cells and their precursors, except mature erythrocytes and platelets.