Anti-Hepatitis C, E2 (Clone HEPC-74) - Purified No Carrier Protein - 1 mg

B, ELISA, EM, FC, N

2 - 8°C Wet Ice

Hepatitis C (HCV) is a flavivirus that annually infects an estimated 58 million people worldwide, causing liver failure and cancer 1. HCV has two surface glycoproteins, E1 and E2, that associateto form a heterodimer, E1E2, on the surface of the virion. E1 is thought to function in viral-hostmembrane fusion during entry, whereas E2 is known to interact with the cellular entry receptorstetraspanin CD81, scavenger receptor-B1, occludin, and claudin. E1 and E2 are single-passtransmembrane proteins that do not share sequence or structural similarity with other flavivirustype II fusion glycoproteins. Both E1 and E2 are heavily glycosylated.HEPC-74 was generated by screening donor plasma from patients who had spontaneouslycleared HCV infection for HCV-neutralizing antibodies using a panel of 19 genotype 1a and 1bHCV pseudoparticles 2. Plasma taken just before infection clearance was used in the screening.Peripheral blood mononuclear cells from serum obtained after clearance were then isolated, andB-cells were transformed with EBV, CpG and supplements, cultured, and screened forneutralizing activity against autologous and heterologous HCV variants. Cells capable ofneutralizing HCV were fused with HMMA2.5 myeloma cells for hybridoma production andscreened against HCV E1E2 heterodimer. The resulting monoclonal antibodies were thenpurified and tested for neutralizing breadth against the original 19 genotype panel. HEPC-74 wasfound to neutralize 17/19 variants. Additionally, HEPC-74 neutralized all except genotype 3 in apanel of genotype 1-6 replication-competent cell culture viruses.HEPC-74 binds to a conserved, conformational epitope in the front layer of E2 2 near N-linkedglycans at positions N430 and N448 1. HEPC-74 recognizes E2 using a disulfide-linked CDRH3motif and predominantly binds V H domain residues 3. HEPC-74 blocks CD81 binding to E1E2due to overlapping binding sites 1, 3, 4. HEPC-74 blocks HCV entry at an early post-attachment step, before claudin engagement 4. HEPC-74 binds native E1E2 but not denatured protein 2.HEPC-74 is a neutralizing antibody 4 that can be useful in cryo-EM mapping of E1E2 1.

≥1.0 mg/ml

E2 is encoded by the hepatitis C virus.